Technologies Used In Development Of Immunocontrace

Technologies Used In Development Of Immuno protective Vaccines.

BY December, 2001 Table of mend: 1. Abstr human action....3 2. Introduction..3 2.1 What is Immunocontraception and why?...3 2.2         The Zona Pellucida: as vaccine purport...5 2.3         Sperm surface antigens as vaccine target......5 2.4         Hormones as targets for vaccines..6 3. Methods and techniques.7 3.1 Antiserum production.7 3.2 Adjuvants....9 3.3 speech communication sytems......10 3.4 ELISA...11 3.5 Immunoblotting (Western blotting)......12 3.6 Immunoelectrophoresis and Immunodiffusion..12 3.7 Radio immunoassay.14 4. Discussion...14                   5. References.....15 Abstract.

The normal approach for individuals wishing to control the number and time of pregnancy is by using conventional contraceptives such(prenominal) as; The Pill, condoms, surgical sterilization, injectable hormones and chemical spermicides, just to mention a few. Research institutions around the world are now canvass a new approach for controlling the process of fertilization. By utilizing and stimulating the soundboxs own immune reaction, they are making come up in the exploitation of anti-fertility vaccines, also known as immunocontraceptives.



The development of anti-fertility vaccines are not only envisaged for use in gentleman only, but will also be important in controlling wild life populations, such as to limit the detrimental effects pest species have on their surroundings.

         presently there are three main approaches to the development of contraceptive vaccines, all of which aim to turn the bodys immune body to act against reproductive components. One approach is to induce an immune response against hormones controlling the development of the embryo, such as (hCG). The other two main approaches are the induction of immunity against gamete-specific antigens found on the surface on spermatozoan or the zona pellucida (ZP), a glycoprotein layer that surrounds the egg cell (Aitken, R. J., et al, 1993). With other words, the immune system ability to recognize and ignore self-molecules has to be reprogrammed/manipulated in such a way, to mount an immune response aimed at molecules the body normally tolerates.

        In this review, the main focus has been placed on some of the key laboratory...

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